High-performance, two-step/Bi-enzyme lactate biofuel cell with lactate oxidase and pyruvate oxidase

L-Lactate oxidase

o f c y t o c h r o m e

Determination of serum lactate with alkylamine glass bound lactate oxidase.

Commercial lactate oxidase (Lactate:O2; oxidoreductase EC 1.1.3.2) from Pediococcus species was immobilized on to alkylamine glass beads (pore diameter 55 nm) through glutaraldehyde coupling with a conjugation yield of 3.2 mg/g support and 105% retention of initial activity. Immobilized enzyme showed maximum activity at pH 6.5, when incubated at 40 degrees C for 12 min and was used for determin...

Studies on lactate oxidase substituted with synthetic flavins. Iso-FMN lactate oxidase.

Concerted action of lactate oxidase and pyruvate oxidase in aerobic growth of Streptococcus pneumoniae: role of lactate as an energy source.

Streptococcus pneumoniae was shown to possess lactate oxidase in addition to well-documented pyruvate oxidase. The activities of both H(2)O(2)-forming oxidases in wild-type cultures were detectable even in the early exponential phase of growth and attained the highest levels in the early stationary phase. For each of these oxidases, a defective mutant was constructed and compared to the parent ...

Stabilization of Lactate Oxidase Flavin Anion Radical

The red-colored flavin anion radical of lactate oxidase was formed by photochemical reduction. The radical was found to be reoxidized readily by molecular oxygen, with a rate constant of 4 X lo5 M "I min" at pH 7.0, 25°C. On mixing the radical under anaerobic conditions with pyruvate, a change in spectrum typical of charge transfer interaction was found. The complex was composed of 1 eq of pyru...